C-terminal region of apoptin affects chicken anemia virus replication and virulence
نویسندگان
چکیده
منابع مشابه
Chicken anemia virus.
Chicken anemia virus is commonly found in commercially produced chickens and has a worldwide distribution. It is difficult to inactivate thermally or with common disinfectants, which limits the utility of normal sanitization practices. The virus is important because of the disease it produces following transovarian transmission and because of its potential for inducing immunosuppression alone o...
متن کاملCloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus
Chicken anemia virus was detected by PCR in tissue samples collected from poultry flocks in Gujarat,India. The VP1, VP2 and VP3 gene sequences of CAV from Anand, Gujarat were obtained after cloning thePCR products in pDrive cloning vector. Nucleotide sequence alignment with other CAV sequencesdemonstrated overall identity of 95-98.8%, 98.8-99.8% and 98.8-100% for VP1, VP2 and VP3 regions,respec...
متن کاملProkaryotic expression of chicken infectious anemia apoptin protein and characterization of its polyclonal antibodies.
In the present study recombinant VP3 (rVP3) was expressed in E. coli BL21 (DE3) (pLysS) and its polyclonal antibodies were characterized. SDS-PAGE analysis revealed that the expression of recombinant protein was maximum when induced with 1.5 mM IPTG for 6 h at 37 degrees C. The 6xHis-tagged fusion protein was purified on Ni-NTA and confirmed by Western blot using CAV specific antiserum. Rabbits...
متن کاملThe chicken anemia virus-derived protein apoptin requires activation of caspases for induction of apoptosis in human tumor cells.
The chicken anemia virus protein Apoptin has been shown to induce apoptosis in a large number of transformed and tumor cell lines, but not in primary cells. Whereas many other apoptotic stimuli (e.g., many chemotherapeutic agents and radiation) require functional p53 and are inhibited by Bcl-2, Apoptin acts independently of p53, and its activity is enhanced by Bcl-2. Here we study the involveme...
متن کاملEfficient Production of an Engineered Apoptin from Chicken Anemia Virus in a Recombinant E. coli for Tumor Therapeutic Applications
BACKGROUND Apoptin, a nonstructural protein encoded by the VP3 gene of chicken anemia virus (CAV), has been shown to not only induce apoptosis when introduced into the precursors of chicken thymocytes, but has been found to specifically kill human cancer cells, tumor cell and transformed cells without affecting the proliferation of normal cells. This tumor-specific apoptotic characteristic of t...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2017
ISSN: 1743-422X
DOI: 10.1186/s12985-017-0713-9